The future war represented by high-precision guided weapons and unmanned systems has a high demand for satellite navigation signals. Based on the theory of satellite navigation spoofing interference, the concept of agile navigation warfare supported by low-orbit constellation is proposed. The definition and connotation of the concept are given, and its winning mechanism is further explained. By installing spoofing interference equipment, signal suppression and spoofing with more maneuverability, flexibility, and wider coverage can be achieved. Based on the concept of agile navigation warfare, specific combat scenarios are designed from the aspects of combat objectives, battlefield environment, combat forces, combat operations, combat effects, etc. With multi-means support in the sea, land, air and other combat domains, through adaptive control of spoofing signal power and dynamic adjustment of spoofing strategies, it ensures continuous and stable spoofing interference signal output, achieving regional signal suppression of blue threat targets and gradual covert inductive spoofing and deviation. This provides capability to support the red side to obtain the battlefield initiative and effectively carry out defense and attack missions. The key technologies that need tobe broken through in the implementation of agile navigation warfare and their feasibility are analyzed and explained.